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COX2/PTGS2 Rabbit mAb (A3560)

Review (1)Publications (27) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - COX2/PTGS2 Rabbit mAb (A3560)

Western blot analysis of various lysates using COX2/PTGS2 Rabbit mAb (A3560) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Immunohistochemistry - COX2/PTGS2 Rabbit mAb (A3560)

Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma tissue using COX2/PTGS2 Rabbit mAb (A3560) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - COX2/PTGS2 Rabbit mAb (A3560)

Immunohistochemistry analysis of paraffin-embedded Human colon tissue using COX2/PTGS2 Rabbit mAb (A3560) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - COX2/PTGS2 Rabbit mAb (A3560)

Immunohistochemistry analysis of paraffin-embedded Mouse intestin tissue using COX2/PTGS2 Rabbit mAb (A3560) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - COX2/PTGS2 Rabbit mAb (A3560)

Immunohistochemistry analysis of paraffin-embedded Rat colon tissue using COX2/PTGS2 Rabbit mAb (A3560) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Review
ABclonal: review for COX2/PTGS2 Rabbit mAb(A3560)

Overview

Product nameCOX2/PTGS2 Rabbit mAb
Catalog No.A3560
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC0800
Prostaglandin-endoperoxide synthase (PTGS), also known as cyclooxygenase, is the key enzyme in prostaglandin biosynthesis, and acts both as a dioxygenase and as a peroxidase. There are two isozymes of PTGS: a constitutive PTGS1 and an inducible PTGS2, which differ in their regulation of expression and tissue distribution. This gene encodes the inducible isozyme. It is regulated by specific stimulatory events, suggesting that it is responsible for the prostanoid biosynthesis involved in inflammation and mitogenesis.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 505-604 of human COX2/PTGS2 (P35354).
SequenceGETMVEVGAPFSLKGLMGNVICSPAYWKPSTFGGEVGFQIINTASIQSLICNNVKGCPFTSFSVPDPELIKTVTINASSSRSGLDDINPTVLLKERSTEL
Gene ID
Swiss Prot
SynonymsCOX2; COX-2; PHS-2; PGG/HS; PGHS-2; hCox-2; GRIPGHS; COX2/PTGS2
Calculated MW69kDa
Observed MW72kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:1000 - 1:6000
  • IHC-P 1:200 - 1:2000
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    
Positive samplesHeLa+LPS, RAW 264.7+LPS, Rat brain
Cellular locationcaveola, cytoplasm, endoplasmic reticulum, endoplasmic reticulum lumen, neuron projection.
Customer validation

(Hela,NIH/3T3)

WB(Rattus norvegicus, Mus musculus, Homo sapiens, Mus musculus, Gallus gallus)

IHC(Mus musculus, Homo sapiens, Rattus norvegicus)

IHC(Mus musculus)

IHC(Homo sapiens, Mus musculus, Rattus norvegicus)

IF(Rattus norvegicus)

ELISA(Rattus norvegicus)

IF(Mus musculus)

Other(Mus musculus)

Documents

Certificate of Compliance

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ABclonal:Western blot - COX2/PTGS2 Rabbit mAb (A3560)}

Western blot - COX2/PTGS2 Rabbit mAb (A3560)

Western blot analysis of various lysates using COX2/PTGS2 Rabbit mAb (A3560) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
ABclonal:Immunohistochemistry - COX2/PTGS2 Rabbit mAb (A3560)}

Immunohistochemistry - COX2/PTGS2 Rabbit mAb (A3560)

Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma tissue using COX2/PTGS2 Rabbit mAb (A3560) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - COX2/PTGS2 Rabbit mAb (A3560)}

Immunohistochemistry - COX2/PTGS2 Rabbit mAb (A3560)

Immunohistochemistry analysis of paraffin-embedded Human colon tissue using COX2/PTGS2 Rabbit mAb (A3560) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - COX2/PTGS2 Rabbit mAb (A3560)}

Immunohistochemistry - COX2/PTGS2 Rabbit mAb (A3560)

Immunohistochemistry analysis of paraffin-embedded Mouse intestin tissue using COX2/PTGS2 Rabbit mAb (A3560) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - COX2/PTGS2 Rabbit mAb (A3560)}

Immunohistochemistry - COX2/PTGS2 Rabbit mAb (A3560)

Immunohistochemistry analysis of paraffin-embedded Rat colon tissue using COX2/PTGS2 Rabbit mAb (A3560) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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Reviews List

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항체 (3)

재조합 단백질 (1)

ELISA 키트 (1)

Secondary Antibodies (26)