제품 > 항체 > 다중클론항체(pAb)

β-Catenin Rabbit pAb (A0316)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

Western blot analysis of lysates from Mouse lung, using β-Catenin Rabbit pAb (A0316) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Immunoprecipitation - β-Catenin Rabbit pAb (A0316)

Immunoprecipitation analysis of 600 μg extracts of Mouse brain using 3 μg β-Catenin antibody (A0316). Western blot was performed from the immunoprecipitate using β-Catenin antibody (A0316) at a dilution of 1:1000.

Overview

Product name	β-Catenin Rabbit pAb
Catalog No.	A0316
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

The protein encoded by this gene is part of a complex of proteins that constitute adherens junctions (AJs). AJs are necessary for the creation and maintenance of epithelial cell layers by regulating cell growth and adhesion between cells. The encoded protein also anchors the actin cytoskeleton and may be responsible for transmitting the contact inhibition signal that causes cells to stop dividing once the epithelial sheet is complete. Finally, this protein binds to the product of the APC gene, which is mutated in adenomatous polyposis of the colon. Mutations in this gene are a cause of colorectal cancer (CRC), pilomatrixoma (PTR), medulloblastoma (MDB), and ovarian cancer. Alternative splicing results in multiple transcript variants.

Immunogen information

Immunogen	A synthetic peptide corresponding to a sequence within amino acids 1-100 of human β-Catenin (NP_001895.1).
Sequence	GVATYAAAVLFRMSEDKPQDYKKRLSVELTSSLFRTEPMAWNETADLGLDIGAQGEPLGYRQDDPSYRSFHSGGYGQDALGMDPMMEHEMGGHHPGADYPV
Gene ID
Swiss Prot
Synonyms	EVR7; CTNNB; MRD19; NEDSDV; armadillo; β-Catenin
Calculated MW	85kDa
Observed MW	100kDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:1000 IHC-P 1:50 - 1:200 IP 0.5μg-4μg antibody for 400μg-600μg extracts of whole cells ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key application	Western blotting Immunohistochemistry Immunoprecipitation
Positive samples	Mouse lung, Mouse brain
Cellular location	Cell junction, Cell membrane, Cytoplasm, Nucleus, adherens junction, centrosome, cytoskeleton, microtubule organizing center, spindle pole.
Customer validation	WB(Homo sapiens, Rattus norvegicus, Rattus norvegicus, Mus musculus, Other) IHC(Rattus norvegicus) WB(Rattus norvegicus) IHC(Other) IF(Homo sapiens)

Documents

Certificate of Compliance

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Lot number

Research Area

β-Catenin Rabbit pAb images

Western blot - β-Catenin Rabbit pAb (A0316)

Immunoprecipitation - β-Catenin Rabbit pAb (A0316)

* For research use only. Not for therapeutic or diagnostic purposes.

Product Protocols

References for β-Catenin Rabbit pAb (A0316)

Product:

β-Catenin Rabbit pAb

Journal:

Journal Of Translational Medicine

Application:

IF:

8.4

Species:

Homo sapiens

PMID:

26541651

Title:

Targeting protein arginine methyltransferase 5 inhibits human hepatocellular carcinoma growth via the downregulation of beta-catenin

References for β-Catenin Rabbit pAb (A0316)

Product:

β-Catenin Rabbit pAb

Journal:

TISSUE ENGINEERING PART A

Application:

IF:

3.5

Species:

Rattus norvegicus

PMID:

28107808

Title:

In Situ Release of VEGF Enhances Osteogenesis in 3D Porous Scaffolds Engineered with Osterix-Modified Adipose-Derived Stem Cells

References for β-Catenin Rabbit pAb (A0316)

Product:

β-Catenin Rabbit pAb

Journal:

Aging

Application:

IF:

3.9

Species:

Homo sapiens

PMID:

36750173

Title:

miRNA-874-3p inhibits the migration, invasion and proliferation of breast cancer cells by targeting VDAC1 - PMC

References for β-Catenin Rabbit pAb (A0316)

Product:

β-Catenin Rabbit pAb

Journal:

Research (Washington, D.C.)

Application:

IF:

Species:

Homo sapiens

PMID:

37746658

Title:

The Mechanics of Tumor Cells Dictate Malignancy via Cytoskeleton-Mediated APC/Wnt/β-Catenin Signaling - PMC

References for β-Catenin Rabbit pAb (A0316)

Product:

β-Catenin Rabbit pAb

Journal:

International immunopharmacology

Application:

IF:

4.8

Species:

Homo sapiens, Rattus norvegicus

PMID:

37567011

Title:

Restoration of miR-328a-5p function curtails hypoxic pulmonary hypertension through a mechanism involving PIN1/GSK3/-catenin axis

References for β-Catenin Rabbit pAb (A0316)

Product:

β-Catenin Rabbit pAb

Journal:

Molecular medicine (Cambridge, Mass.)

Application:

IHC, WB

IF:

Species:

Rattus norvegicus

PMID:

38395742

Title:

WSB1, as an E3 ligase, restrains myocardial ischemia-reperfusion injury by activating -catenin signaling via promoting GSK3 ubiquitination - PMC

References for β-Catenin Rabbit pAb (A0316)

Product:

β-Catenin Rabbit pAb

Journal:

Elife

Application:

IF:

6.4

Species:

Mus musculus

PMID:

32186512

Title:

Piezo1/2 mediate mechanotransduction essential for bone formation through concerted activation of NFAT-YAP1--catenin

References for β-Catenin Rabbit pAb (A0316)

Product:

β-Catenin Rabbit pAb

Journal:

Fitoterapia

Application:

WB, IHC

IF:

2.5

Species:

Other

PMID:

37751799

Title:

Hydroalcoholic root extracts of Houttuynia cordata (Thunb.) standardized by UPLC-Q-TOF-MS/MS promotes apoptosis in human hepatocarcinoma cell HepG2 via

References for β-Catenin Rabbit pAb (A0316)

Product:

β-Catenin Rabbit pAb

Journal:

Journal Of The Neurological Sciences

Application:

WB, IF

IF:

3.6

Species:

Homo sapiens

PMID: