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[KO Validated] APP Rabbit mAb (A17911)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - [KO Validated] APP Rabbit mAb (A17911)

Western blot analysis of lysates from wild type (WT) and APP knockout (KO) 293T cells, using [KO Validated] APP Rabbit mAb (A17911) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

Western blot analysis of various lysates using [KO Validated] APP Rabbit mAb (A17911) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Immunofluorescence - [KO Validated] APP Rabbit mAb (A17911)

Immunofluorescence analysis of HeLa cells using APP Rabbit mAb (A17911) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Confocal imaging of paraffin-embedded 5XFAD Mouse brain and Mouse brain using [KO Validated] APP Rabbit mAb (A17911, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x.

ABclonal:Immunoprecipitation - [KO Validated] APP Rabbit mAb (A17911)

Immunoprecipitation analysis of 300 μg extracts from HeLa cells using 3 μg [KO Validated] APP Rabbit mAb (A17911). Western blot was performed from the immunoprecipitate using [KO Validated] APP Rabbit mAb (A17911) at a dilution of 1:1000.

Overview

Product name	[KO Validated] APP Rabbit mAb
Catalog No.	A17911
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG
CloneNo.	ARC0465

Background

This gene encodes a cell surface receptor and transmembrane precursor protein that is cleaved by secretases to form a number of peptides. Some of these peptides are secreted and can bind to the acetyltransferase complex APBB1/TIP60 to promote transcriptional activation, while others form the protein basis of the amyloid plaques found in the brains of patients with Alzheimer disease. In addition, two of the peptides are antimicrobial peptides, having been shown to have bacteriocidal and antifungal activities. Mutations in this gene have been implicated in autosomal dominant Alzheimer disease and cerebroarterial amyloidosis (cerebral amyloid angiopathy). Multiple transcript variants encoding several different isoforms have been found for this gene.

Immunogen information

Immunogen	A synthetic peptide corresponding to a sequence within amino acids 671-770 of human APP (P05067).
Sequence	MDAEFRHDSGYEVHHQKLVFFAEDVGSNKGAIIGLMVGGVVIATVIVITLVMLKKKQYTSIHHGVVEVDAAVTPEERHLSKMQQNGYENPTYKFFEQMQN
Gene ID
Swiss Prot
Synonyms	AAA; AD1; PN2; ABPP; APPI; CVAP; ABETA; PN-II; preA4; CTFgamma; alpha-sAPP; PP
Calculated MW	87kDa
Observed MW	100-140kDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:1000 - 1:6000 IF/ICC 1:100 - 1:800 IP 0.5μg-4μg antibody for 200μg-400μg extracts of whole cells ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key application	Western blotting Immunofluorescence Immunoprecipitation
Positive samples	293T, HeLa, U-87 MG, Mouse brain, Rat brain
Cellular location	Membrane, Single-pass type I membrane protein, clathrin-coated pit.
Customer validation	IF(Rattus norvegicus) WB(Homo sapiens, Rattus norvegicus, Mus musculus) IF(Mus musculus) IHC(Rattus norvegicus)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

Research Area

[KO Validated] APP Rabbit mAb images

Western blot - [KO Validated] APP Rabbit mAb (A17911)

Immunofluorescence - [KO Validated] APP Rabbit mAb (A17911)

Immunoprecipitation - [KO Validated] APP Rabbit mAb (A17911)

* For research use only. Not for therapeutic or diagnostic purposes.

Product Protocols

References for [KO Validated] APP Rabbit mAb (A17911)

Product:

[KO Validated] APP Rabbit mAb

Journal:

Neurochem Int

Application:

IF:

3.92

Species:

Rattus norvegicus

PMID:

34048842

Title:

Lovastatin attenuates sevoflurane-induced cognitive disorder in aged rats via reducing Aβ accumulation

References for [KO Validated] APP Rabbit mAb (A17911)

Product:

[KO Validated] APP Rabbit mAb

Journal:

International journal of molecular sciences

Application:

IF:

6.2

Species:

Homo sapiens

PMID:

36430913

Title:

dNAGLU Extends Life Span and Promotes Fitness and Stress Resistance in Drosophila - PMC

References for [KO Validated] APP Rabbit mAb (A17911)

Product:

[KO Validated] APP Rabbit mAb

Journal:

Translational stroke research

Application:

IF:

6.8

Species:

Rattus norvegicus

PMID:

36577854

Title:

A Novel Hybrid of Telmisartan and Borneol Ameliorates Neuroinflammation and White Matter Injury in Ischemic Stroke Through ATF3/CH25H Axis

References for [KO Validated] APP Rabbit mAb (A17911)

Product:

[KO Validated] APP Rabbit mAb

Journal:

Bioorganic chemistry

Application:

IF:

5.3

Species:

Mus musculus

PMID:

36868127

Title:

Design, synthesis and bioevaluation of 1, 2, 4-thiadiazolidine-3, 5-dione derivatives as potential GSK-3β inhibitors for the treatment of Alzheimer's disease

References for [KO Validated] APP Rabbit mAb (A17911)

Product:

[KO Validated] APP Rabbit mAb

Journal:

International journal of biological sciences

Application:

IF:

9.2

Species:

Mus musculus

PMID:

38481801

Title:

mtDNA-cGAS-STING axis-dependent NLRP3 inflammasome activation contributes to postoperative cognitive dysfunction induced by sevoflurane in mice

References for [KO Validated] APP Rabbit mAb (A17911)

Product:

[KO Validated] APP Rabbit mAb

Journal:

Food Funct

Application:

WB, IF

IF:

6.317

Species:

Mus musculus

PMID:

34676845

Title:

Protective effect and mechanism of docosahexaenoic acid on the cognitive function in female APP/PS1 mice

References for [KO Validated] APP Rabbit mAb (A17911)

Product:

[KO Validated] APP Rabbit mAb

Journal:

Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association

Application:

IF:

5.57

Species:

Mus musculus

PMID:

35697184

Title:

Silver nanoparticles induced hippocampal neuronal damage involved in mitophagy, mitochondrial biogenesis and synaptic degeneration

References for [KO Validated] APP Rabbit mAb (A17911)

Product:

[KO Validated] APP Rabbit mAb

Journal:

Inflammopharmacology

Application:

IHC

IF:

5.09

Species:

Rattus norvegicus

PMID:

37460908

Title:

Neuromodulatory effect of vardenafil on aluminium chloride/d-galactose induced Alzheimer's disease in rats: emphasis on amyloid-beta, p-tau, PI3K/Akt/p53

References for [KO Validated] APP Rabbit mAb (A17911)

Product:

[KO Validated] APP Rabbit mAb

Journal:

Frontiers in pharmacology

Application:

IF:

5.988

Species:

Homo sapiens,Rattus norvegicus

PMID:

35462916

Title:

Pharmacodynamics, Network Pharmacology, and Pharmacokinetics of Chinese Medicine Formula 9002A in the Treatment of Alzheimer's Disease

References for [KO Validated] APP Rabbit mAb (A17911)

Product:

[KO Validated] APP Rabbit mAb

Journal:

Translational psychiatry

Application:

IF:

6.8

Species:

Mus musculus

PMID:

38796566

Title:

Oxytocin attenuates hypothalamic injury-induced cognitive dysfunction by inhibiting hippocampal ERK signaling and A deposition - PMC

References for [KO Validated] APP Rabbit mAb (A17911)

Product:

[KO Validated] APP Rabbit mAb

Journal:

Molecules (Basel, Switzerland)

Application:

IF:

4.6

Species:

Rattus norvegicus

PMID:

38792167

Title:

Synergistic Effect of Flavonoids and Metformin on Protection of the Methylglyoxal-Induced Damage in PC-12 Neuroblastoma Cells: Structure-Activity Relationship and Potential Target - PMC

References for [KO Validated] APP Rabbit mAb (A17911)

Product:

[KO Validated] APP Rabbit mAb

Journal:

Frontiers in pharmacology

Application:

IF:

5.6

Species:

Rattus norvegicus

PMID:

39101143

Title:

Melatonin modulates TLR4/MyD88/NF-B signaling pathway to ameliorate cognitive impairment in sleep-deprived rats - PMC

References for [KO Validated] APP Rabbit mAb (A17911)

Product:

[KO Validated] APP Rabbit mAb

Journal:

Frontiers in pharmacology

Application:

IF:

5.6

Species:

Rattus norvegicus

PMID:

39101143

Title:

Melatonin modulates TLR4/MyD88/NF-B signaling pathway to ameliorate cognitive impairment in sleep-deprived rats - PMC

References for [KO Validated] APP Rabbit mAb (A17911)

Product:

[KO Validated] APP Rabbit mAb

Journal:

Translational stroke research

Application:

IF:

6.9

Species:

Mus musculus

PMID:

38861152

Title:

Single-Cell Transcriptomics Revealed White Matter Repair Following Subarachnoid Hemorrhage

References for [KO Validated] APP Rabbit mAb (A17911)

Product:

[KO Validated] APP Rabbit mAb

Journal:

Pharmaceuticals (Basel, Switzerland)

Application:

IHC

IF:

4.6

Species:

Rattus norvegicus

PMID:

39204160

Title:

Nifedipine Improves the Ketogenic Diet Effect on Insulin-Resistance-Induced Cognitive Dysfunction in Rats - PMC

References for [KO Validated] APP Rabbit mAb (A17911)

Product:

[KO Validated] APP Rabbit mAb

Journal:

Biomedicines

Application:

IF:

4.7

Species:

Mus musculus

PMID:

39200168

Title:

A High-Carbohydrate Diet Induces Cognitive Impairment and Promotes Amyloid Burden and Tau Phosphorylation via PI3K/Akt/GSK-3 Pathway in db/db Mice - PMC