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PE/Cyanine7 Rabbit anti-Human CD25 mAb (A27400)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human

ABclonal:Flow CytoMetry - PE/Cyanine7 Rabbit anti-Human CD25 mAb (A27400)

Flow cytometry: 1X10^6 Human PBMC were surface-stained with PE/Cyanine7 Rabbit IgG isotype control (5 μl/Test, left) or PE/Cyanine7 Rabbit anti-Human CD25 mAb (A27400, 5 μl/Test, right). Cells in the Lymphocyte gate were used for analysis.

Overview

Product namePE/Cyanine7 Rabbit anti-Human CD25 mAb
Catalog No.A27400
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC58045-PE-Cy7
The interleukin 2 (IL2) receptor alpha (IL2RA) and beta (IL2RB) chains, together with the common gamma chain (IL2RG), constitute the high-affinity IL2 receptor. Homodimeric alpha chains (IL2RA) result in low-affinity receptor, while homodimeric beta (IL2RB) chains produce a medium-affinity receptor. Normally an integral-membrane protein, soluble IL2RA has been isolated and determined to result from extracellular proteolyisis. Alternately-spliced IL2RA mRNAs have been isolated, but the significance of each is presently unknown. Mutations in this gene are associated with interleukin 2 receptor alpha deficiency. Patients with severe Coronavirus Disease 2019 (COVID-19), the disease caused by the novel severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), have significantly elevated levels of IL2R in their plasma. Similarly, serum IL-2R levels are found to be elevated in patients with different types of carcinomas. Certain IL2RA and IL2RB gene polymorphisms have been associated with lung cancer risk.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 22-213 of human CD25 (NP_000408.1).
SequenceELCDDDPPEIPHATFKAMAYKEGTMLNCECKRGFRRIKSGSLYMLCTGNSSHSSWDNQCQCTSSATRNTTKQVTPQPEEQKERKTTEMQSPMQPVDQASLPGHCREPPPWENEATERIYHFVVGQMVYYQCVQGYRALHRGPAESVCKMTHGKTRWTQPQLICTGEMETSQFPGEEKPQASPEGRPESETSC
Gene ID
Swiss Prot
Synonymsp55; CD25; IL2R; IMD41; TCGFR; IDDM10
Calculated MW31kDa
Observed MW
ReactivityHuman
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • FC 5 μl per 10^6 cells in 100 μl volume
Storage bufferStore at 2-8℃. Avoid freeze.
Buffer: PBS with 0.09% Sodium azide, 0.2% BSA, pH7.3.
Key applicationFlow Cytometry    
Positive samples
Cellular locationPlasma membrane.

Documents

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ABclonal:Flow CytoMetry - PE/Cyanine7 Rabbit anti-Human CD25 mAb (A27400)}

Flow CytoMetry - PE/Cyanine7 Rabbit anti-Human CD25 mAb (A27400)

Flow cytometry: 1X10^6 Human PBMC were surface-stained with PE/Cyanine7 Rabbit IgG isotype control (5 μl/Test, left) or PE/Cyanine7 Rabbit anti-Human CD25 mAb (A27400, 5 μl/Test, right). Cells in the Lymphocyte gate were used for analysis.

* For research use only. Not for therapeutic or diagnostic purposes.

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