제품 > 항체 > 다중클론항체(pAb)

NF-kB p65/RelA Rabbit pAb (A11201)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Rat

Western blot analysis of various lysates using NF-kB p65/RelA Rabbit pAb (A11201) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

Western blot analysis of lysates from Rat lung , using NF-kB p65/RelA Rabbit pAb (A11201) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Immunofluorescence - NF-kB p65/RelA Rabbit pAb (A11201)

Immunofluorescence analysis of C6 cells using NF-kB p65/RelA Rabbit pAb (A11201) at dilution of 1:200 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product name	NF-kB p65/RelA Rabbit pAb
Catalog No.	A11201
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

NF-kappa-B is a ubiquitous transcription factor involved in several biological processes. It is held in the cytoplasm in an inactive state by specific inhibitors. Upon degradation of the inhibitor, NF-kappa-B moves to the nucleus and activates transcription of specific genes. NF-kappa-B is composed of NFKB1 or NFKB2 bound to either REL, RELA, or RELB. The most abundant form of NF-kappa-B is NFKB1 complexed with the product of this gene, RELA. Four transcript variants encoding different isoforms have been found for this gene.

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 450-550 of human NF-kB p65/RelA (NP_068810.3).
Sequence	LGALLGNSTDPAVFTDLASVDNSEFQQLLNQGIPVAPHTTEPMLMEYPEAITRLVTGAQRPPDPAPAPLGAPGLPNGLLSGDEDFSSIADMDFSALLSQIS
Gene ID
Swiss Prot
Synonyms	p65; CMCU; NFKB3; AIF3BL3; NF-kB p65/RelA
Calculated MW	60kDa
Observed MW	65kDa

Applications

Reactivity	Human, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:1000 IHC-P 1:50 - 1:200 IF/ICC 1:50 - 1:200
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key application	Western blotting Immunohistochemistry Immunofluorescence
Positive samples	HeLa, 293T, Rat lung
Cellular location	Cytoplasm, Nucleus
Customer validation	WB(Mus musculus, Gallus gallus, Sus scrofa, Rattus norvegicus, Homo sapiens) IF(Homo sapiens) IP(Sus scrofa) IF(Rattus norvegicus)

Documents

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Research Area

NF-kB p65/RelA Rabbit pAb images

Western blot - NF-kB p65/RelA Rabbit pAb (A11201)

Immunofluorescence - NF-kB p65/RelA Rabbit pAb (A11201)

* For research use only. Not for therapeutic or diagnostic purposes.

Product Protocols

References for NF-kB p65/RelA Rabbit pAb (A11201)

Product:

NF-kB p65/RelA Rabbit pAb

Journal:

Scientific reports

Application:

IF:

3.8

Species:

Mus musculus

PMID:

28916796

Title:

Low temperature plasma promoting fibroblast proliferation by activating the NF-κB pathway and increasing cyclinD1 expression

References for NF-kB p65/RelA Rabbit pAb (A11201)

Product:

NF-kB p65/RelA Rabbit pAb

Journal:

Cells

Application:

IF:

7.7

Species:

Gallus gallus

PMID:

36139393

Title:

Extracellular HMGB1 as Inflammatory Mediator in the Progression of Mycoplasma Gallisepticum Infection - PMC

References for NF-kB p65/RelA Rabbit pAb (A11201)

Product:

NF-kB p65/RelA Rabbit pAb

Journal:

Experimental and therapeutic medicine

Application:

IF:

2.4

Species:

Homo sapiens

PMID:

37090067

Title:

SHFM1 deficiency suppresses esophageal squamous cell carcinomas progression via modulating NF-κB signaling and enhancing nature killer cell-mediated tumor surveillance - PMC

References for NF-kB p65/RelA Rabbit pAb (A11201)

Product:

NF-kB p65/RelA Rabbit pAb

Journal:

Molecules (Basel, Switzerland)

Application:

IF:

4.6

Species:

Mus musculus

PMID:

38005205

Title:

Zaluzanin C Alleviates Inflammation and Lipid Accumulation in Kupffer Cells and Hepatocytes by Regulating Mitochondrial ROS - PMC

References for NF-kB p65/RelA Rabbit pAb (A11201)

Product:

NF-kB p65/RelA Rabbit pAb

Journal:

PLoS pathogens

Application:

IF:

5.5

Species:

Sus scrofa

PMID:

36215225

Title:

miRNA let-7 family regulated by NEAT1 and ARID3A/NF-B inhibits PRRSV-2 replication in vitro and in vivo - PMC

References for NF-kB p65/RelA Rabbit pAb (A11201)

Product:

NF-kB p65/RelA Rabbit pAb

Journal:

PLoS pathogens

Application:

IF:

5.5

Species:

Sus scrofa

PMID:

36215225

Title:

miRNA let-7 family regulated by NEAT1 and ARID3A/NF-B inhibits PRRSV-2 replication in vitro and in vivo - PMC

References for NF-kB p65/RelA Rabbit pAb (A11201)

Product:

NF-kB p65/RelA Rabbit pAb

Journal:

Neuropharmacology

Application:

WB, IF

IF:

4.6

Species:

Rattus norvegicus

PMID:

35926582

Title:

Pharmacological inhibition of the cGAS-STING signaling pathway suppresses microglial M1-polarization in the spinal cord and attenuates neuropathic pain

References for NF-kB p65/RelA Rabbit pAb (A11201)

Product:

NF-kB p65/RelA Rabbit pAb

Journal:

The Science of the total environment

Application:

IF:

8.2

Species:

Homo sapiens

PMID:

38754514