제품 > 항체 > 다중클론항체(pAb)

NF-kB p65/RelA Rabbit pAb (A16271)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

Western blot analysis of various lysates using NF-kB p65/RelA Rabbit pAb (A16271) at 1:500 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3s.

ABclonal:Immunofluorescence - NF-kB p65/RelA Rabbit pAb (A16271)

Immunofluorescence analysis of L929 cells using NF-kB p65/RelA Rabbit pAb (A16271) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product name	NF-kB p65/RelA Rabbit pAb
Catalog No.	A16271
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

NF-kappa-B is a ubiquitous transcription factor involved in several biological processes. It is held in the cytoplasm in an inactive state by specific inhibitors. Upon degradation of the inhibitor, NF-kappa-B moves to the nucleus and activates transcription of specific genes. NF-kappa-B is composed of NFKB1 or NFKB2 bound to either REL, RELA, or RELB. The most abundant form of NF-kappa-B is NFKB1 complexed with the product of this gene, RELA. Four transcript variants encoding different isoforms have been found for this gene.

Immunogen information

Immunogen	A synthetic peptide corresponding to a sequence within amino acids 100-200 of human NF-kB p65/RelA (NP_068810.3).
Sequence	YEAELCPDRCIHSFQNLGIQCVKKRDLEQAISQRIQTNNNPFQVPIEEQRGDYDLNAVRLCFQVTVRDPSGRPLRLPPVLSHPIFDNRAPNTAELKICRVN
Gene ID
Swiss Prot
Synonyms	p65; CMCU; NFKB3; AIF3BL3; NF-kB p65/RelA
Calculated MW	60kDa
Observed MW	65kDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:2000 IF/ICC 1:50 - 1:200
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key application	Western blotting Immunofluorescence
Positive samples	HeLa, A-549, MCF7, Mouse lung, Mouse spleen, Rat lung
Cellular location	Cytoplasm, Nucleus.
Customer validation	WB(Mus musculus, Rattus norvegicus, Homo sapiens) IF(Mus musculus)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

Research Area

NF-kB p65/RelA Rabbit pAb images

Western blot - NF-kB p65/RelA Rabbit pAb (A16271)

Immunofluorescence - NF-kB p65/RelA Rabbit pAb (A16271)

* For research use only. Not for therapeutic or diagnostic purposes.

Product Protocols

References for NF-kB p65/RelA Rabbit pAb (A16271)

Product:

NF-kB p65/RelA Rabbit pAb

Journal:

Food & Function

Application:

IF:

5.1

Species:

Mus musculus

PMID:

32208477

Title:

Fermented blueberry pomace ameliorates intestinal barrier function through the NF-κB-MLCK signaling pathway in high-fat diet mice

References for NF-kB p65/RelA Rabbit pAb (A16271)

Product:

NF-kB p65/RelA Rabbit pAb

Journal:

Biomedicine & Pharmacotherapy

Application:

IF:

6.9

Species:

Mus musculus

PMID:

33197761

Title:

NaoXinTong Capsule ameliorates memory deficit in APP/PS1 mice by regulating inflammatory cytokines

References for NF-kB p65/RelA Rabbit pAb (A16271)

Product:

NF-kB p65/RelA Rabbit pAb

Journal:

Oxidative medicine and cellular longevity

Application:

IF:

Species:

Rattus norvegicus

PMID:

36820405

Title:

Ferroptosis in Rat Lung Tissue during Severe Acute Pancreatitis-Associated Acute Lung Injury: Protection of Qingyi Decoction - PMC

References for NF-kB p65/RelA Rabbit pAb (A16271)

Product:

NF-kB p65/RelA Rabbit pAb

Journal:

MedComm

Application:

IF:

9.9

Species:

Mus musculus

PMID:

37426678

Title:

Identification of circulating T-cell immunoglobulin and mucin domain 4 as a potential biomarker for coronary heart disease - PMC

References for NF-kB p65/RelA Rabbit pAb (A16271)

Product:

NF-kB p65/RelA Rabbit pAb

Journal:

Journal of inflammation research

Application:

IF:

4.5

Species:

Rattus norvegicus

PMID:

36120185

Title:

C/EBP Promotes LPS-Induced IL-1 Transcription and Secretion in Alveolar Macrophages via NOD2 Signaling - PMC

References for NF-kB p65/RelA Rabbit pAb (A16271)

Product:

NF-kB p65/RelA Rabbit pAb

Journal:

Small (Weinheim an der Bergstrasse, Germany)

Application:

IF:

Species:

Homo sapiens

PMID:

38009480

Title:

MnAntiCTLA4CREKASericin Nanotheragnostics for Enhanced Magnetic Resonance Imaging and Tumor Immunotherapy

References for NF-kB p65/RelA Rabbit pAb (A16271)

Product:

NF-kB p65/RelA Rabbit pAb

Journal:

Heliyon

Application:

IF:

Species:

Homo sapiens

PMID:

38163179

Title:

Therapeutic potential of anti-PIK3CG treatment for multiple myeloma via inhibiting c-Myc pathway - PMC

References for NF-kB p65/RelA Rabbit pAb (A16271)

Product:

NF-kB p65/RelA Rabbit pAb

Journal:

Front Aging Neurosci

Application:

WB, IF

IF:

4.1

Species:

Mus musculus

PMID:

33328962

Title:

LongShengZhi Capsule Attenuates Alzheimer-Like Pathology in APP/PS1 Double Transgenic Mice by Reducing Neuronal Oxidative Stress and Inflammation

References for NF-kB p65/RelA Rabbit pAb (A16271)

Product:

NF-kB p65/RelA Rabbit pAb

Journal:

Nutrients

Application:

IF:

5.9

Species:

Mus musculus

PMID:

37432306

Title:

Glabridin Ameliorates Alcohol-Caused Liver Damage by Reducing Oxidative Stress and Inflammation via p38 MAPK/Nrf2/NF-B Pathway - PMC

References for NF-kB p65/RelA Rabbit pAb (A16271)

Product:

NF-kB p65/RelA Rabbit pAb

Journal:

Bioorganic chemistry

Application:

WB, IF

IF:

4.5

Species:

Mus musculus

PMID:

37300959

Title:

Novel flavonoid 1, 3, 4-oxadiazole derivatives ameliorate MPTP-induced Parkinson's disease via Nrf2/NF-B signaling pathway

Publishing research using A16271? Please let us know so that we can cite the reference in this datasheet.

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제품 > 항체 > 다중클론항체(pAb)

NF-kB p65/RelA Rabbit pAb (A16271)

Overview

Background

Immunogen information

Applications

Documents

Certificate of Compliance

Research Area

NF-kB p65/RelA Rabbit pAb images

Product Protocols

항체 (15)

재조합 단백질 (1)

Secondary Antibodies (25)