제품 > 항체 > 인산화 특이적 항체

Phospho-NF-kB p65/RelA-S536 Rabbit pAb (AP0475)

Review (1)Publications (64) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - Phospho-NF-kB p65/RelA-S536 Rabbit pAb (AP0475)

Western blot analysis of lysates from HeLa cells using Phospho-NF-kB p65/RelA-S536 Rabbit pAb (AP0475) at 1:1000 dilution. HeLa cells were treated by TNF-α (20 ng/mL) at 37℃ for 30 minutes.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

ABclonal:Western blot - Phospho-NF-kB p65/RelA-S536 Rabbit pAb (AP0475)

Western blot analysis of lysates from NIH/3T3 cells using Phospho-NF-kB p65/RelA-S536 Rabbit pAb (AP0475) at 1:600 dilution. NIH/3T3 cells were treated by TNF-α (20 ng/mL) at 37℃ for 30 minutes.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

ABclonal:Western blot - Phospho-NF-kB p65/RelA-S536 Rabbit pAb (AP0475)

Western blot analysis of lysates from C6 cells using Phospho-NF-kB p65/RelA-S536 Rabbit pAb (AP0475) at 1:600 dilution. C6 cells were treated by TNF-α (20 ng/mL) at 37℃ for 30 minutes.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

ABclonal:Immunohistochemistry - Phospho-NF-kB p65/RelA-S536 Rabbit pAb (AP0475)

Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using Phospho-NF-kB p65/RelA-S536 Rabbit pAb (AP0475) at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - Phospho-NF-kB p65/RelA-S536 Rabbit pAb (AP0475)

Immunohistochemistry analysis of paraffin-embedded Rat kidney using Phospho-NF-kB p65/RelA-S536 Rabbit pAb (AP0475) at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Review
ABclonal: review for Phospho-NF-kB p65/RelA-S536 Rabbit pAb(AP0475)

Overview

Product namePhospho-NF-kB p65/RelA-S536 Rabbit pAb
Catalog No.AP0475
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
NF-kappa-B is a ubiquitous transcription factor involved in several biological processes. It is held in the cytoplasm in an inactive state by specific inhibitors. Upon degradation of the inhibitor, NF-kappa-B moves to the nucleus and activates transcription of specific genes. NF-kappa-B is composed of NFKB1 or NFKB2 bound to either REL, RELA, or RELB. The most abundant form of NF-kappa-B is NFKB1 complexed with the product of this gene, RELA. Four transcript variants encoding different isoforms have been found for this gene.
ImmunogenA synthetic phosphorylated peptide around S536 of human NF-kB p65/RelA (NP_068810.3).
SequenceFSSIA
Gene ID
Swiss Prot
Synonymsp65; CMCU; NFKB3; AIF3BL3; Phospho-NF-kB p65/RelA-S536
Calculated MW60kDa
Observed MW65kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:50 - 1:200
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    
Positive samplesHeLa treated by TNF-α, NIH/3T3 treated by TNF-α, C6 treated by TNF-α
Cellular locationCytoplasm, Nucleus.
Customer validation

(Raw264.7)

WB(Mus musculus, Homo sapiens, Rattus norvegicus, Bos taurus, Mus musculus, Rattus norvegicus, Other)

IHC(Mus musculus)

IF(Homo sapiens, Mus musculus)

IHC(Homo sapiens, Mus musculus)

ChIP(Mus musculus)

IF(Rattus norvegicus)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

ABclonal:Western blot - Phospho-NF-kB p65/RelA-S536 Rabbit pAb (AP0475)}

Western blot - Phospho-NF-kB p65/RelA-S536 Rabbit pAb (AP0475)

Western blot analysis of lysates from HeLa cells using Phospho-NF-kB p65/RelA-S536 Rabbit pAb (AP0475) at 1:1000 dilution. HeLa cells were treated by TNF-α (20 ng/mL) at 37℃ for 30 minutes.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.
ABclonal:Western blot - Phospho-NF-kB p65/RelA-S536 Rabbit pAb (AP0475)}

Western blot - Phospho-NF-kB p65/RelA-S536 Rabbit pAb (AP0475)

Western blot analysis of lysates from NIH/3T3 cells using Phospho-NF-kB p65/RelA-S536 Rabbit pAb (AP0475) at 1:600 dilution. NIH/3T3 cells were treated by TNF-α (20 ng/mL) at 37℃ for 30 minutes.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.
ABclonal:Western blot - Phospho-NF-kB p65/RelA-S536 Rabbit pAb (AP0475)}

Western blot - Phospho-NF-kB p65/RelA-S536 Rabbit pAb (AP0475)

Western blot analysis of lysates from C6 cells using Phospho-NF-kB p65/RelA-S536 Rabbit pAb (AP0475) at 1:600 dilution. C6 cells were treated by TNF-α (20 ng/mL) at 37℃ for 30 minutes.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.
ABclonal:Immunohistochemistry - Phospho-NF-kB p65/RelA-S536 Rabbit pAb (AP0475)}

Immunohistochemistry - Phospho-NF-kB p65/RelA-S536 Rabbit pAb (AP0475)

Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using Phospho-NF-kB p65/RelA-S536 Rabbit pAb (AP0475) at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - Phospho-NF-kB p65/RelA-S536 Rabbit pAb (AP0475)}

Immunohistochemistry - Phospho-NF-kB p65/RelA-S536 Rabbit pAb (AP0475)

Immunohistochemistry analysis of paraffin-embedded Rat kidney using Phospho-NF-kB p65/RelA-S536 Rabbit pAb (AP0475) at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

* For research use only. Not for therapeutic or diagnostic purposes.

Submit an review for AP0475

Please submit reviews to your technical sales specialist directly. Alternatively, email us at info@abclonal.co.kr

Reviews List

Publishing research using AP0475? Please let us know so that we can cite the reference in this datasheet.

항체 (15)

재조합 단백질 (1)

Secondary Antibodies (25)