제품 > 항체 > 인산화 특이적 항체

Phospho-STAT3-S727 Rabbit pAb (AP0474)

Publications (10) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - Phospho-STAT3-S727 Rabbit pAb (AP0474)

Western blot analysis of lysates from HeLa cells, using Phospho-STAT3-S727 Rabbit pAb (AP0474) at 1:1000 dilution. HeLa cells were treated by UV at room temperature for 15-30 minutes. HeLa cells were treated by PMA/TPA (200 nM) at 37℃ for 15 minutes after serum-starvation overnight.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Western blot - Phospho-STAT3-S727 Rabbit pAb (AP0474)

Western blot analysis of various lysates using Phospho-STAT3-S727 Rabbit pAb (AP0474) at 1:1000 dilution. HepG2 cells and C6 cells were treated by PMA/TPA (200 nM) at 37℃ for 30 minutes after serum-starvation overnight. HepG2 cells were treated by CIP(20uL/400ul) at 37℃ for 1 hour.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Immunohistochemistry - Phospho-STAT3-S727 Rabbit pAb (AP0474)

Immunohistochemistry analysis of paraffin-embedded Rat lung using Phospho-STAT3-S727 Rabbit pAb (AP0474) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.

ABclonal:Immunohistochemistry - Phospho-STAT3-S727 Rabbit pAb (AP0474)

Immunohistochemistry analysis of paraffin-embedded Human breast cancer using Phospho-STAT3-S727 Rabbit pAb (AP0474) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.

ABclonal:Immunohistochemistry - Phospho-STAT3-S727 Rabbit pAb (AP0474)

Immunohistochemistry analysis of paraffin-embedded Mouse lung using Phospho-STAT3-S727 Rabbit pAb (AP0474) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.

ABclonal:Immunoprecipitation - Phospho-STAT3-S727 Rabbit pAb (AP0474)

Immunoprecipitation analysis of 200 μg extracts of HeLa cells, using 3 μg Phospho-STAT3-S727 pAb (AP0474). Western blot was performed from the immunoprecipitate using Phospho-STAT3-S727 pAb (AP0474) at a dilution of 1:1000. HeLa cells were treated by PMA/TPA (200 nM) at 37℃ for 15 minutes after serum-starvation overnight.

Overview

Product namePhospho-STAT3-S727 Rabbit pAb
Catalog No.AP0474
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is activated through phosphorylation in response to various cytokines and growth factors including IFNs, EGF, IL5, IL6, HGF, LIF and BMP2. This protein mediates the expression of a variety of genes in response to cell stimuli, and thus plays a key role in many cellular processes such as cell growth and apoptosis. The small GTPase Rac1 has been shown to bind and regulate the activity of this protein. PIAS3 protein is a specific inhibitor of this protein. This gene also plays a role in regulating host response to viral and bacterial infections. Mutations in this gene are associated with infantile-onset multisystem autoimmune disease and hyper-immunoglobulin E syndrome.
ImmunogenA synthetic phosphorylated peptide around S727 of human Phospho-STAT3-S727 (NP_644805.1).
SequencePMSPR
Gene ID
Swiss Prot
SynonymsAPRF; HIES; ADMIO; ADMIO1; Phospho-STAT3-S727
Calculated MW88kDa
Observed MW86kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:50 - 1:200
  • IP 0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.09% Sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunoprecipitation    
Positive samplesHeLa, HeLa treated by UV or PMA, HepG2, HepG2 treated by PMA, C6 treated by UV or PMA
Cellular locationCytoplasm, Nucleus.
Customer validation

IF(Mus musculus)

IHC(Gallus gallus)

WB(Homo sapiens, Ovis aries, Mus musculus)

FC(Mus musculus)

IP(Homo sapiens)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

ABclonal:Western blot - Phospho-STAT3-S727 Rabbit pAb (AP0474)}

Western blot - Phospho-STAT3-S727 Rabbit pAb (AP0474)

Western blot analysis of lysates from HeLa cells, using Phospho-STAT3-S727 Rabbit pAb (AP0474) at 1:1000 dilution. HeLa cells were treated by UV at room temperature for 15-30 minutes. HeLa cells were treated by PMA/TPA (200 nM) at 37℃ for 15 minutes after serum-starvation overnight.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
ABclonal:Western blot - Phospho-STAT3-S727 Rabbit pAb (AP0474)}

Western blot - Phospho-STAT3-S727 Rabbit pAb (AP0474)

Western blot analysis of various lysates using Phospho-STAT3-S727 Rabbit pAb (AP0474) at 1:1000 dilution. HepG2 cells and C6 cells were treated by PMA/TPA (200 nM) at 37℃ for 30 minutes after serum-starvation overnight. HepG2 cells were treated by CIP(20uL/400ul) at 37℃ for 1 hour.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
ABclonal:Immunohistochemistry - Phospho-STAT3-S727 Rabbit pAb (AP0474)}

Immunohistochemistry - Phospho-STAT3-S727 Rabbit pAb (AP0474)

Immunohistochemistry analysis of paraffin-embedded Rat lung using Phospho-STAT3-S727 Rabbit pAb (AP0474) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.
ABclonal:Immunohistochemistry - Phospho-STAT3-S727 Rabbit pAb (AP0474)}

Immunohistochemistry - Phospho-STAT3-S727 Rabbit pAb (AP0474)

Immunohistochemistry analysis of paraffin-embedded Human breast cancer using Phospho-STAT3-S727 Rabbit pAb (AP0474) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.
ABclonal:Immunohistochemistry - Phospho-STAT3-S727 Rabbit pAb (AP0474)}

Immunohistochemistry - Phospho-STAT3-S727 Rabbit pAb (AP0474)

Immunohistochemistry analysis of paraffin-embedded Mouse lung using Phospho-STAT3-S727 Rabbit pAb (AP0474) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.
ABclonal:Immunoprecipitation - Phospho-STAT3-S727 Rabbit pAb (AP0474)}

Immunoprecipitation - Phospho-STAT3-S727 Rabbit pAb (AP0474)

Immunoprecipitation analysis of 200 μg extracts of HeLa cells, using 3 μg Phospho-STAT3-S727 pAb (AP0474). Western blot was performed from the immunoprecipitate using Phospho-STAT3-S727 pAb (AP0474) at a dilution of 1:1000. HeLa cells were treated by PMA/TPA (200 nM) at 37℃ for 15 minutes after serum-starvation overnight.

* For research use only. Not for therapeutic or diagnostic purposes.

Publishing research using AP0474? Please let us know so that we can cite the reference in this datasheet.

항체 (8)

STAT3 Rabbit pAb
A1192

WBIHC-PIF/ICCELISA

Reactivity:Human, Mouse, Rat

STAT3 Rabbit pAb

Phospho-STAT3-Y705 Rabbit pAb
AP0070

ELISAWBIHC-P

Reactivity:Human, Mouse, Rat

Phospho-STAT3-Y705 Rabbit pAb

Phospho-STAT3-Y705 Rabbit mAb
AP0705

WBIHC-PIF/ICCELISA

Reactivity:Human, Mouse, Rat

Phospho-STAT3-Y705 Rabbit mAb

[KO Validated] STAT3 Rabbit mAb
A19566

WBIHC-PELISA

Reactivity:Human, Mouse, Rat

[KO Validated] STAT3 Rabbit mAb

Phospho-STAT3-S727 Rabbit mAb
AP0715

ELISAWBIHC-P

Reactivity:Human, Mouse, Rat

Phospho-STAT3-S727 Rabbit mAb

STAT3 Rabbit mAb
A22434

WBIF/ICCIPELISA

Reactivity:Human, Mouse, Rat

STAT3 Rabbit mAb

STAT3 Rabbit pAb
A11216

ELISAWBIF/ICC

Reactivity:Human, Mouse, Rat

STAT3 Rabbit pAb

STAT3 Rabbit pAb
A16975

ELISAWB

Reactivity:Human, Mouse, Rat

STAT3 Rabbit pAb

ELISA 키트 (1)

Human Signal transducer and activator of transcription 3 (STAT3) ELISA Kit
RK02341

Human Signal transducer and activator of transcription 3 (STAT3) ELISA Kit

재조합 단백질 (1)

Recombinant Human STAT3 Protein
RP00447

Tags:C-His

Expressed Host:E. coli

Recombinant Human STAT3 Protein

Secondary Antibodies (26)

HRP-conjugated Goat anti-Rabbit IgG (H+L)
AS014

WBDBIHC-PELISA

HRP-conjugated Goat anti-Rabbit IgG (H+L)

FITC-conjugated Goat anti-Rabbit IgG (H+L)
AS011

IF/ICCFC

FITC-conjugated Goat anti-Rabbit IgG (H+L)

Cy3-conjugated Goat anti-Rabbit IgG (H+L)
AS007

IF/ICCFC

Cy3-conjugated Goat anti-Rabbit IgG (H+L)

ABflo® 488-conjugated Goat anti-Rabbit IgG (H+L)
AS053

IF/ICCFC

ABflo® 488-conjugated Goat anti-Rabbit IgG (H+L)

HRP-conjugated Mouse anti-Rabbit IgG Light Chain
AS061

WBIP

HRP-conjugated Mouse anti-Rabbit IgG Light Chain

HRP-conjugated Goat anti-Rabbit IgG Heavy Chain
AS063

WB

HRP-conjugated Goat anti-Rabbit IgG Heavy Chain

ABflo® 594-conjugated Goat anti-Rabbit IgG (H+L)
AS039

IF/ICCFC

ABflo® 594-conjugated Goat anti-Rabbit IgG (H+L)

Rhodamine (TRITC)-conjugated Goat anti-Rabbit IgG (H+L)
AS040

IHC-PIF/ICCFC

Rhodamine (TRITC)-conjugated Goat anti-Rabbit IgG (H+L)

ABflo® 647-conjugated Goat anti-Rabbit IgG (H+L)
AS060

IF/ICCFC

ABflo® 647-conjugated Goat anti-Rabbit IgG (H+L)

Goat Anti-Rabbit IgG(H+L)
AS070

WBELISACUT&Tag

Goat Anti-Rabbit IgG(H+L)

HRP-conjugated Donkey anti-Rabbit IgG (H+L)
AS038

WBIHC-P

HRP-conjugated Donkey anti-Rabbit IgG (H+L)

ABflo® 488-conjugated Donkey anti-Rabbit IgG (H+L)
AS035

IF/ICCFC

ABflo® 488-conjugated Donkey anti-Rabbit IgG (H+L)

ABflo® 488-conjugated Goat anti-Rabbit IgG (H+L)
AS073

IF/ICCFC

ABflo® 488-conjugated Goat anti-Rabbit IgG (H+L)

ABflo® 555-conjugated Goat anti-Rabbit IgG (H+L)
AS058

IF/ICCFC

ABflo® 555-conjugated Goat anti-Rabbit IgG (H+L)

FITC-conjugated Donkey anti-Rabbit IgG (H+L)
AS042

IF/ICCFC

FITC-conjugated Donkey anti-Rabbit IgG (H+L)

ABflo® 405-conjugated Goat anti-Rabbit IgG (H+L)
AS056

IF/ICCFC

ABflo® 405-conjugated Goat anti-Rabbit IgG (H+L)

AP-conjugated Goat anti-Rabbit IgG (H+L)
AS009

WB

AP-conjugated Goat anti-Rabbit IgG (H+L)

HRP-conjugated Mouse anti-Rabbit IgG (H+L)
AS079

WB

HRP-conjugated Mouse anti-Rabbit IgG (H+L)

HRP-conjugated Mouse anti-Rabbit IgG Light Chain mAb
AS126

IPELISA

HRP-conjugated Mouse anti-Rabbit IgG Light Chain mAb

PE Mouse anti-Rabbit IgG, light chain specific mAb
AS129

FC

PE Mouse anti-Rabbit IgG, light chain specific mAb

HRP conjugated Mouse anti-Rabbit IgG Heavy Chain mAb
AS122

WBELISA

HRP conjugated Mouse anti-Rabbit IgG Heavy Chain mAb

Rabbit Anti-Chicken IgY (H+L)
AS090

WB

Rabbit Anti-Chicken IgY (H+L)

Rabbit Anti-Chicken IgY Fab
AS088

WB

Rabbit Anti-Chicken IgY Fab

FITC-conjugated F(ab')₂ Fragment Goat anti-Rabbit IgG, Fc fragment specific
AS083

IF/ICCFC

FITC-conjugated F(ab')₂ Fragment Goat anti-Rabbit IgG, Fc fragment specific

Rabbit Anti-Chicken IgY (H+L)
AS082

WB

Rabbit Anti-Chicken IgY (H+L)

ABflo® 750-conjugated Goat anti-Rabbit IgG (H+L)
AS072

IF/ICCFC

ABflo® 750-conjugated Goat anti-Rabbit IgG (H+L)