제품 > 항체 > 단일클론항체(mAb)

ABflo® 488 Streptavidin (AT0001)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Species independent

Flow cytometry: 1X10^6 Jurkat cells (negative control, left) and U-2 OS cells (right) were surface-stained with Biotin Rabbit anti-Human CD40 mAb (A25703, 2 μg/mL, orange line) or Biotin Rabbit IgG isotype control (A25626, 2 μg/mL, blue line), followed by ABflo® 488 Streptavidin(AT0001, 5 μl/Test) staining. Non-fluorescently stained cells were used as blank control (red line).

Flow cytometry: 1X10^6 U-2 OS cells were surface-stained with Biotin Rabbit IgG isotype control (A25626, 2 μg/mL, blue line) or Biotin Rabbit anti-Human CD40 mAb (A25703, 2 μg/mL, right), followed by ABflo® 488 Streptavidin(AT0001, 5 μl/Test) staining.

Overview

Product name	ABflo® 488 Streptavidin
Catalog No.	AT0001
Host species
Purification method
Isotype
CloneNo.	Streptavidin-ABf488

Background

Streptavidin binds to biotin with high affinity. Streptavidin-ABflo® 488 is useful for detecting biotinylated antibodies.

Immunogen information

Immunogen
Sequence
Gene ID
Swiss Prot
Synonyms	SA V1; SA V2
Calculated MW
Observed MW

Applications

Reactivity	Species independent
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	FC 5 μl per 10^6 cells in 100 μl volume
Storage buffer	Store at 2-8℃. Avoid freeze. Buffer: PBS with 0.03% proclin300, 0.2% BSA, pH7.3.
Key application	Flow Cytometry
Positive samples
Cellular location	.